Chomczynski, P., & Sacchi, N. (1987). Single-step method of RNA 
isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. 
Analytical biochemistry, 162(1), 156-159.

Abstract
A new method of total RNA isolation by a single extraction with an acid 
guanidinium thiocyanate-phenol-chloroform mixture is described. The 
method provides a pure preparation of undegraded RNA in high yield and 
can be completed within 4 h. It is particularly useful for processing large 
numbers of samples and for isolation of RNA from minute quantities of cells or
tissue samples.